› 6dej

X-ray diffraction
1.63Å resolution

The structure of HcRed7, a brighter and red-shifted HcRed variant

Released:
DOI: 10.2210/pdb6dej/pdb
PDB entry 6dej coloured by chain, front view.
PDB entry 6dej coloured by chain, side view.
PDB entry 6dej coloured by chain, top view.
Source organism: Heteractis crispa
Primary publication:
Monomerization of far-red fluorescent proteins.
Wannier TM, Gillespie SK, Hutchins N, McIsaac RS, Wu SY, Shen Y, Campbell RE, Brown KS, Mayo SL
Proc Natl Acad Sci U S A 115 E11294-E11301 (2018)
PMID: 30425172

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-188277 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GFP-like non-fluorescent chromoprotein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 235 amino acids
Theoretical weight: 26.83 KDa
Source organism: Heteractis crispa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q95W85 (Residues: 1-227; Coverage: 100%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

3 bound ligands:
Ligand SO4 2 x SO4
Ligand 12P 1 x 12P
Ligand CL 1 x CL
1 modified residue:
Ligand CRU 4 x CRU

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P21
Unit cell:
a: 54.342Å b: 122.057Å c: 75.254Å
α: 90° β: 108.75° γ: 90°
R-values:
R R work R free
0.178 0.178 0.214
Expression system: Escherichia coli

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Citations

2 review citations

Bacterial Vivisection: How Fluorescence-Based Imaging Techniques Shed a Light on the Inner Workings of Bacteria.
Cambré et al. (2020)
1 more

1 mention without citation

Monomerization of far-red fluorescent proteins.
Wannier et al. (2018)