› 6dfk

X-ray diffraction
3.1Å resolution

Crystal structure of the 11S subunit of the Plasmodium falciparum proteasome, PA28

Released:
DOI: 10.2210/pdb6dfk/pdb
PDB entry 6dfk coloured by chain, front view.
PDB entry 6dfk coloured by chain, side view.
PDB entry 6dfk coloured by chain, top view.
Source organism: Plasmodium falciparum 3D7
Primary publication:
The structure of the PA28-20S proteasome complex from Plasmodium falciparum and implications for proteostasis.
Xie SC, Metcalfe RD, Hanssen E, Yang T, Gillett DL, Leis AP, Morton CJ, Kuiper MJ, Parker MW, Spillman NJ, Wong W, Tsu C, Dick LR, Griffin MDW, Tilley L
Nat Microbiol 4 1990-2000 (2019)
PMID: 31384003

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184655 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proteasome activator PA28 C-terminal domain-containing protein Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Length: 280 amino acids
Theoretical weight: 33.24 KDa
Source organism: Plasmodium falciparum 3D7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8I374 (Residues: 1-279; Coverage: 100%)
Gene name: PF3D7_0907700
Sequence domains: Proteasome activator pa28 beta subunit

Ligands and Environments

1 bound ligand:
Ligand SO4 60 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P3121
Unit cell:
a: 166.485Å b: 166.485Å c: 399.162Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.182 0.225
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

MalDA, Accelerating Malaria Drug Discovery.
Yang et al. (2021)
2 more

1 mention without citation

Structure, Function, and Allosteric Regulation of the 20S Proteasome by the 11S/PA28 Family of Proteasome Activators.
Thomas et al. (2023)