› 6dhv

X-ray diffraction
2.1Å resolution

Structure of Arabidopsis Fatty Acid Amide Hydrolase

Released:
DOI: 10.2210/pdb6dhv/pdb
PDB entry 6dhv coloured by chain, front view.
PDB entry 6dhv coloured by chain, side view.
PDB entry 6dhv coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural analysis of a plant fatty acid amide hydrolase provides insights into the evolutionary diversity of bioactive acylethanolamides.
Aziz M, Wang X, Tripathi A, Bankaitis VA, Chapman KD
J Biol Chem 294 7419-7432 (2019)
PMID: 30894416

Function and Biology Details

Reaction catalysed: 
Anandamide + H(2)O = arachidonic acid + ethanolamine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182192 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fatty acid amide hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 636 amino acids
Theoretical weight: 69.58 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7XJJ7 (Residues: 1-607; Coverage: 100%)
Gene names: At5g64440, FAAH, T12B11.3
Sequence domains: Amidase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL14-1
Spacegroup: P21
Unit cell:
a: 72.083Å b: 79.66Å c: 132.592Å
α: 90° β: 104.44° γ: 90°
R-values:
R R work R free
0.193 0.192 0.22
Expression system: Escherichia coli

Quick links

Citations

1 review citation

GPR119 and GPR55 as Receptors for Fatty Acid Ethanolamides, Oleoylethanolamide and Palmitoylethanolamide.
Im DS. (2021)
4 other articles

4 mentions without citation

Beyond the Usual Suspects: Physiological Roles of the Arabidopsis Amidase Signature (AS) Superfamily Members in Plant Growth Processes and Stress Responses.
Moya-Cuevas et al. (2021)
3 more