› 6dhw

X-ray diffraction
2.01Å resolution

Crystal structure of primase iron-sulfur domain (266-457)

Released:
DOI: 10.2210/pdb6dhw/pdb
PDB entry 6dhw coloured by chain, front view.
PDB entry 6dhw coloured by chain, side view.
PDB entry 6dhw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Functional and structural similarity of human DNA primase [4Fe4S] cluster domain constructs.
Holt ME, Salay LE, O'Brien E, Barton JK, Chazin WJ
PLoS One 13 e0209345 (2018)
PMID: 30562384

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155976 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA primase large subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 192 amino acids
Theoretical weight: 22.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49643 (Residues: 266-457; Coverage: 38%)
Gene names: PRIM2, PRIM2A
Sequence domains: Eukaryotic and archaeal DNA primase, large subunit

Ligands and Environments

2 bound ligands:
Ligand SF4 2 x SF4
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: C2
Unit cell:
a: 110.193Å b: 52.56Å c: 88.774Å
α: 90° β: 115.08° γ: 90°
R-values:
R R work R free
0.182 0.181 0.211
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

DNA primase large subunit is an essential plant gene for geminiviruses, putatively priming viral ss-DNA replication.
Siskos et al. (2023)
2 more