› 6dii

X-ray diffraction
3.2Å resolution

Structure of Arabidopsis Fatty Acid Amide Hydrolase in Complex with methyl linolenyl fluorophosphonate

Released:
DOI: 10.2210/pdb6dii/pdb
PDB entry 6dii coloured by chain, front view.
PDB entry 6dii coloured by chain, side view.
PDB entry 6dii coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural analysis of a plant fatty acid amide hydrolase provides insights into the evolutionary diversity of bioactive acylethanolamides.
Aziz M, Wang X, Tripathi A, Bankaitis VA, Chapman KD
J Biol Chem 294 7419-7432 (2019)
PMID: 30894416

Function and Biology Details

Reaction catalysed: 
Anandamide + H(2)O = arachidonic acid + ethanolamine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182192 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fatty acid amide hydrolase Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 636 amino acids
Theoretical weight: 69.58 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7XJJ7 (Residues: 1-607; Coverage: 100%)
Gene names: At5g64440, FAAH, T12B11.3
Sequence domains: Amidase

Ligands and Environments

1 bound ligand:
Ligand GJY 12 x GJY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL14-1
Spacegroup: P21
Unit cell:
a: 225.577Å b: 83.311Å c: 272.811Å
α: 90° β: 110.98° γ: 90°
R-values:
R R work R free
0.255 0.252 0.296
Expression system: Escherichia coli

Quick links

Citations

2 review citations

GPR119 and GPR55 as Receptors for Fatty Acid Ethanolamides, Oleoylethanolamide and Palmitoylethanolamide.
Im DS. (2021)
1 more

4 mentions without citation

Structural analysis of a plant fatty acid amide hydrolase provides insights into the evolutionary diversity of bioactive acylethanolamides.
Aziz et al. (2019)
3 more