› 6dj4

X-ray diffraction
3.01Å resolution

Crystal Structure of Bacillus thuringiensis Cry1A.105 Tryptic Core

Released:
DOI: 10.2210/pdb6dj4/pdb
PDB entry 6dj4 coloured by chain, front view.
PDB entry 6dj4 coloured by chain, side view.
PDB entry 6dj4 coloured by chain, top view.
Source organism: Bacillus thuringiensis serovar aizawai
Primary publication:
Safety of the Bacillus thuringiensis-derived Cry1A.105 protein: Evidence that domain exchange preserves mode of action and safety.
Wang C, Li W, Kessenich CR, Petrick JS, Rydel TJ, Sturman EJ, Lee TC, Glenn KC, Edrington TC
Regul Toxicol Pharmacol 99 50-60 (2018)
PMID: 30196079

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141163 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pesticidal crystal protein Cry1Ab; Pesticidal crystal protein Cry1Fa Chain: A
Molecule details ›
Chain: A
Length: 591 amino acids
Theoretical weight: 66.54 KDa
Source organism: Bacillus thuringiensis serovar aizawai
Expression system: Bacillus thuringiensis
UniProt:
  • Canonical: P0A372 (Residues: 29-476; Coverage: 39%)
  • Canonical: Q03746 (Residues: 470-612; Coverage: 12%)
Gene names: bt2, cry-1-2, cry1A(b), cry1Ab, cry1Fa, cryIA(b), cryIC1, cryIF, cryIF(a)
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 124.375Å b: 49.441Å c: 103.692Å
α: 90° β: 108.65° γ: 90°
R-values:
R R work R free
0.222 0.219 0.279
Expression system: Bacillus thuringiensis

Quick links

Citations

1 review citation

Research Progress on the Synthetic Biology of Botanical Biopesticides.
Zhao et al. (2022)
4 other articles

2 mentions without citation

Can (We Make) Bacillus thuringiensis Crystallize More Than Its Toxins?
Tetreau et al. (2021)
1 more