› 6dkk

X-ray diffraction
2.7Å resolution

Function and Biology Details

Reaction catalysed: 
Limited hydrolysis of proteins of the neuroexocytosis apparatus, synaptobrevin (also known as neuronal vesicle-associated membrane protein, VAMP), synaptosome-associated protein of 25 kDa (SNAP25) or syntaxin. No detected action on small molecule substrates.
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-144068 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Botulinum neurotoxin A heavy chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 327 amino acids
Theoretical weight: 37.5 KDa
Source organism: Clostridium botulinum A str. ATCC 19397
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DPI0 (Residues: 547-871; Coverage: 25%)
Gene names: atx, bonT, botA
Sequence domains: Clostridium neurotoxin, Translocation domain

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: R32
Unit cell:
a: 167.659Å b: 167.659Å c: 222.834Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.209 0.223
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Toxicology and pharmacology of botulinum and tetanus neurotoxins: an update.
Pirazzini et al. (2022)
2 more

1 mention without citation

Tetanus Toxin cis-Loop Contributes to Light-Chain Translocation.
Zuverink et al. (2020)