6dmy

Electron Microscopy
3.6Å resolution

Cryo-EM structure of human Ptch1 and ShhN complex

Released:
Source organism: Homo sapiens
Related structures: EMD-7968

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-171796 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Protein patched homolog 1 Chain: A
Molecule details ›
Chain: A
Length: 1349 amino acids
Theoretical weight: 150.19 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q13635 (Residues: 1-1305; Coverage: 90%)
Gene names: PTCH, PTCH1
Sequence domains: Patched family
Sonic hedgehog protein N-product Chain: B
Molecule details ›
Chain: B
Length: 177 amino acids
Theoretical weight: 19.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q15465 (Residues: 24-197; Coverage: 40%)
Gene name: SHH
Sequence domains: Hedgehog amino-terminal signalling domain
Structure domains: Muramoyl-pentapeptide Carboxypeptidase; domain 2

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-7968
Expression systems:
  • Homo sapiens
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'