› 6dn0

X-ray diffraction
2Å resolution

Retrofitted antibodies with stabilizing mutations: Herceptin scFv mutant with VH K30D and VL S52D.

Released:
DOI: 10.2210/pdb6dn0/pdb
PDB entry 6dn0 coloured by chain, front view.
PDB entry 6dn0 coloured by chain, side view.
PDB entry 6dn0 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Langley DB, Roome B, Christ D

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-232203 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Human Variable Heavy Chain of Herceptin containing VH mutation K30D Chains: A, C
Molecule details ›
Chains: A, C
Length: 136 amino acids
Theoretical weight: 14.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
Human Variable Light Chain of Herceptin containing VL mutation S52D Chains: B, D
Molecule details ›
Chains: B, D
Length: 106 amino acids
Theoretical weight: 11.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Ligands and Environments

1 bound ligand:
Ligand FMT 4 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P3121
Unit cell:
a: 91.981Å b: 91.981Å c: 114.677Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.205 0.234
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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