› 6dxs

X-ray diffraction
1.65Å resolution

Crystal structure of the LigJ hydratase E284Q mutant substrate complex with (3Z)-2-keto-4-carboxy-3-hexenedioate

Released:
DOI: 10.2210/pdb6dxs/pdb
PDB entry 6dxs coloured by chain, front view.
PDB entry 6dxs coloured by chain, side view.
PDB entry 6dxs coloured by chain, top view.
Source organism: Sphingobium sp. SYK-6
Primary publication:
Structure and Reaction Mechanism of the LigJ Hydratase: An Enzyme Critical for the Bacterial Degradation of Lignin in the Protocatechuate 4,5-Cleavage Pathway.
Hogancamp TN, Mabanglo MF, Raushel FM
Biochemistry 57 5841-5850 (2018)
PMID: 30207699

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124254 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-keto-4-carboxy-3-hexenedioate hydratase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 342 amino acids
Theoretical weight: 38.2 KDa
Source organism: Sphingobium sp. SYK-6
Expression system: Escherichia coli
UniProt:
  • Canonical: G2IQQ5 (Residues: 2-341; Coverage: 100%)
Gene names: SLG_12520, ligJ
Sequence domains: Amidohydrolase

Ligands and Environments

2 bound ligands:
Ligand ZN 4 x ZN
Ligand HJ7 4 x HJ7
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21
Unit cell:
a: 82.689Å b: 78.253Å c: 136.216Å
α: 90° β: 89.98° γ: 90°
R-values:
R R work R free
0.206 0.202 0.266
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Dicer-like proteins influence Arabidopsis root microbiota independent of RNA-directed DNA methylation.
Kaushal et al. (2021)
2 more

1 mention without citation

Structure and Reaction Mechanism of the LigJ Hydratase: An Enzyme Critical for the Bacterial Degradation of Lignin in the Protocatechuate 4,5-Cleavage Pathway.
Hogancamp et al. (2018)