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› 6dz5

X-ray diffraction
2.26Å resolution

Citrobacter freundii tyrosine phenol-lyase F448A mutant complexed with L-alanine

Released:
DOI: 10.2210/pdb6dz5/pdb
Model geometry
Fit model/data
PDB entry 6dz5 coloured by chain, front view.
PDB entry 6dz5 coloured by chain, side view.
PDB entry 6dz5 coloured by chain, top view.
Source organism: Citrobacter freundii
Primary publication:
Crystal Structures of Wild-Type and F448A Mutant Citrobacter freundii Tyrosine Phenol-Lyase Complexed with a Substrate and Inhibitors: Implications for the Reaction Mechanism.
Phillips RS, Craig S
Biochemistry 57 6166-6179 (2018)
PMID: 30260636

Function and Biology Details

Reaction catalysed: 
[L-tyrosine + H2O = phenol + pyruvate + NH4(+)., L-tyrosine + H2O = phenol + pyruvate + NH4(+).]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151875 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine phenol-lyase Chains: A, B
Molecule details ›
Chains: A, B
Length: 456 amino acids
Theoretical weight: 51.43 KDa
Source organism: Citrobacter freundii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31013 (Residues: 1-456; Coverage: 100%)
Gene name: tpl
Sequence domains: Beta-eliminating lyase

Ligands and Environments

3 bound ligands:
Ligand K 2 x K
Ligand P61 2 x P61
Ligand P33 1 x P33
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 22-ID
Unit cell:
a: 59.88Å b: 132.81Å c: 145.37Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.221
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Pancreatic Adenocarcinoma: Unconventional Approaches for an Unconventional Disease.
Gromisch et al. (2020)
2 more