› 6dzm

Electron Microscopy
4.29Å resolution

Bundibugyo virus GP (mucin-deleted) in complex with pan-ebolavirus human antibody ADI-15878 Fab

Released:
DOI: 10.2210/pdb6dzm/pdb
PDB entry 6dzm coloured by chain, front view.
PDB entry 6dzm coloured by chain, side view.
PDB entry 6dzm coloured by chain, top view.
Source organisms:
Primary publication:
Structural Basis of Pan-Ebolavirus Neutralization by an Antibody Targeting the Glycoprotein Fusion Loop.
Murin CD, Bruhn JF, Bornholdt ZA, Copps J, Stanfield R, Ward AB
Cell Rep 24 2723-2732.e4 (2018)
PMID: 30184505
Related structures: EMD-8936

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-207686 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (6 distinct):
Filoviruses glycoprotein extracellular domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 311 amino acids
Theoretical weight: 34.7 KDa
Source organism: Bundibugyo virus
Expression system: Homo sapiens
UniProt:
  • Canonical: B8XCN0 (Residues: 33-312; Coverage: 41%)
Gene names: DF49_53413gpGP, DH33_45404gpGP, GP
Sequence domains: Filovirus glycoprotein
Filoviruses glycoprotein extracellular domain-containing protein Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 177 amino acids
Theoretical weight: 19.68 KDa
Source organism: Bundibugyo virus
Expression system: Homo sapiens
UniProt:
  • Canonical: B8XCN0 (Residues: 502-640, 641-648; Coverage: 22%)
Gene names: DF49_53413gpGP, DH33_45404gpGP, GP
Antibody ADI-15878 Fab, light chain Chains: H, K, L
Molecule details ›
Chains: H, K, L
Length: 106 amino acids
Theoretical weight: 11.33 KDa
Source organism: Homo sapiens
Expression system: Nicotiana benthamiana
Antibody ADI-15878 Fab, heavy chain Chains: G, I, J
Molecule details ›
Chains: G, I, J
Length: 120 amino acids
Theoretical weight: 13.04 KDa
Source organism: Homo sapiens
Expression system: Nicotiana benthamiana

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
1 bound ligand:
Ligand NAG 6 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.29Å
Relevant EMDB volumes: EMD-8936
Expression systems:
  • Homo sapiens
  • Nicotiana benthamiana

Quick links

Citations

4 review citations

Cryo-EM Studies of Virus-Antibody Immune Complexes.
Li et al. (2020)
3 more

5 mentions without citation

Structural Basis of Pan-Ebolavirus Neutralization by an Antibody Targeting the Glycoprotein Fusion Loop.
Murin et al. (2018)
4 more