› 6e1o

Electron Microscopy
3.59Å resolution

afTMEM16 reconstituted in nanodiscs in the presence of Ca2+ and ceramide 24:0

Released:
DOI: 10.2210/pdb6e1o/pdb
PDB entry 6e1o coloured by chain, front view.
PDB entry 6e1o coloured by chain, side view.
PDB entry 6e1o coloured by chain, top view.
Source organism: Aspergillus fumigatus Af293
Primary publication:
Structural basis of Ca2+-dependent activation and lipid transport by a TMEM16 scramblase.
Falzone ME, Rheinberger J, Lee BC, Peyear T, Sasset L, Raczkowski AM, Eng ET, Di Lorenzo A, Andersen OS, Nimigean CM, Accardi A
Elife 8 (2019)
PMID: 30648972
Related structures: EMD-8959

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175798 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Plasma membrane channel protein (Aqy1) Chains: A, B
Molecule details ›
Chains: A, B
Length: 735 amino acids
Theoretical weight: 84.62 KDa
Source organism: Aspergillus fumigatus Af293
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q4WA18 (Residues: 1-735; Coverage: 100%)
Gene name: AFUA_4G02970
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand CA 4 x CA
Ligand D12 8 x D12
Ligand D10 2 x D10
Ligand 8K6 2 x 8K6
Ligand HEX 2 x HEX
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.59Å
Relevant EMDB volumes: EMD-8959
Expression system: Saccharomyces cerevisiae

Quick links

Citations

10 review citations

Structural and functional consequences of reversible lipid asymmetry in living membranes.
Doktorova et al. (2020)
9 more

3 mentions without citation

Structural basis of Ca2+-dependent activation and lipid transport by a TMEM16 scramblase.
Falzone et al. (2019)
2 more