6e5u

X-ray diffraction
3.8Å resolution

Crystal structure of the mRNA export receptor NXF1/NXT1 in complex with influenza virus NS1 protein

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-125233 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Nuclear RNA export factor 1 Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 508 amino acids
Theoretical weight: 57.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UBU9 (Residues: 116-619; Coverage: 81%)
Gene names: NXF1, TAP
Sequence domains:
NTF2-related export protein 1 Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 140 amino acids
Theoretical weight: 15.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UKK6 (Residues: 1-140; Coverage: 100%)
Gene name: NXT1
Sequence domains: Nuclear transport factor 2 (NTF2) domain
Structure domains: Nuclear Transport Factor 2; Chain: A,
Non-structural protein 1 Chains: J, K, L, M
Molecule details ›
Chains: J, K, L, M
Length: 77 amino acids
Theoretical weight: 8.45 KDa
Source organism: Influenza A virus
Expression system: Escherichia coli
UniProt:
  • Canonical: I7CAR2 (Residues: 1-72; Coverage: 31%)
Gene names: NS, NS1
Sequence domains: Influenza non-structural protein (NS1)
Structure domains: S15/NS1, RNA-binding
Non-structural protein 1 Chains: U, V, X, Y
Molecule details ›
Chains: U, V, X, Y
Length: 153 amino acids
Theoretical weight: 17.16 KDa
Source organism: Influenza A virus
Expression system: Escherichia coli
UniProt:
  • Canonical: I7CAR2 (Residues: 73-230; Coverage: 67%)
Gene names: NS, NS1
Sequence domains: Influenza non-structural protein (NS1)
Structure domains: Influenza virus non-structural protein, effector domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P65
Unit cell:
a: 101.905Å b: 101.905Å c: 949.476Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.238 0.236 0.282
Expression system: Escherichia coli