› 6e5w

X-ray diffraction
2.5Å resolution

Crystal structure of human cellular retinol binding protein 3 in complex with abnormal-cannabidiol (abn-CBD)

Released:
DOI: 10.2210/pdb6e5w/pdb
PDB entry 6e5w coloured by chain, front view.
PDB entry 6e5w coloured by chain, side view.
PDB entry 6e5w coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Abnormal Cannabidiol Modulates Vitamin A Metabolism by Acting as a Competitive Inhibitor of CRBP1.
Silvaroli JA, Widjaja-Adhi MAK, Trischman T, Chelstowska S, Horwitz S, Banerjee S, Kiser PD, Blaner WS, Golczak M
ACS Chem Biol 14 434-448 (2019)
PMID: 30721022

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160661 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Retinol-binding protein 5 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 139 amino acids
Theoretical weight: 16.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P82980 (Residues: 1-135; Coverage: 100%)
Gene name: RBP5
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

2 bound ligands:
Ligand HVD 4 x HVD
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 37.46Å b: 127.51Å c: 160.229Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.189 0.238
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

The molecular aspects of absorption and metabolism of carotenoids and retinoids in vertebrates.
Widjaja-Adhi et al. (2020)
3 more

1 mention without citation

Abnormal Cannabidiol Modulates Vitamin A Metabolism by Acting as a Competitive Inhibitor of CRBP1.
Silvaroli et al. (2019)