› 6e62

X-ray diffraction
2.7Å resolution

Crystal structure of malaria transmission-blocking antigen Pfs48/45 6C in complex with antibody 85RF45.1

Released:
DOI: 10.2210/pdb6e62/pdb
Model geometry
Fit model/data
PDB entry 6e62 coloured by chain, front view.
PDB entry 6e62 coloured by chain, side view.
PDB entry 6e62 coloured by chain, top view.
Source organisms:
Primary publication:
Structural delineation of potent transmission-blocking epitope I on malaria antigen Pfs48/45.
Kundu P, Semesi A, Jore MM, Morin MJ, Price VL, Liang A, Li J, Miura K, Sauerwein RW, King CR, Julien JP
OpenAccess logo Nat Commun 9 4458 (2018)
PMID: 30367064

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212582 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Gametocyte surface protein P45/48 Chains: A, P
Molecule details ›
Chains: A, P
Length: 139 amino acids
Theoretical weight: 15.39 KDa
Source organism: Plasmodium falciparum
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8I6T1 (Residues: 291-428; Coverage: 33%)
Gene names: PF13_0247, PF3D7_1346700, PF45/48, PFS45-48, PFS45/48
Sequence domains: Sexual stage antigen s48/45 domain
85RF45.1 Fab heavy chain Chains: B, H
Molecule details ›
Chains: B, H
Length: 222 amino acids
Theoretical weight: 23.94 KDa
Source organism: Rattus norvegicus
Expression system: Homo sapiens
85RF45.1 Fab light chain Chains: C, L

Ligands and Environments

2 bound ligands:
Ligand NAG 2 x NAG
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 54.51Å b: 74.45Å c: 321.89Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.246 0.276
Expression system: Homo sapiens

Quick links

Citations

7 review citations

Immunity against sexual stage Plasmodium falciparum and Plasmodium vivax parasites.
de Jong et al. (2020)
6 more

8 mentions without citation

Structural vaccinology of malaria transmission-blocking vaccines.
Patel et al. (2021)
7 more