› 6e7h

X-ray diffraction
3.3Å resolution

Crystal structure of H5 hemagglutinin mutant Y161A from A/Viet Nam/1203/2004 H5N1 influenza virus in complex with 3'-GcLN

Released:
DOI: 10.2210/pdb6e7h/pdb
PDB entry 6e7h coloured by chain, front view.
PDB entry 6e7h coloured by chain, side view.
PDB entry 6e7h coloured by chain, top view.
Source organism: Influenza A virus (A/Viet Nam/1203/2004(H5N1))
Primary publication:
N-Glycolylneuraminic Acid as a Receptor for Influenza A Viruses.
Broszeit F, Tzarum N, Zhu X, Nemanichvili N, Eggink D, Leenders T, Li Z, Liu L, Wolfert MA, Papanikolaou A, Martínez-Romero C, Gagarinov IA, Yu W, García-Sastre A, Wennekes T, Okamatsu M, Verheije MH, Wilson IA, Boons GJ, de Vries RP
Cell Rep 27 3284-3294.e6 (2019)
PMID: 31189111

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179540 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (5 distinct):
Hemagglutinin Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 334 amino acids
Theoretical weight: 37.86 KDa
Source organism: Influenza A virus (A/Viet Nam/1203/2004(H5N1))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q6DQ33 (Residues: 17-346; Coverage: 58%)
Gene name: HA
Sequence domains: Haemagglutinin
Hemagglutinin HA2 chain Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 177 amino acids
Theoretical weight: 20.41 KDa
Source organism: Influenza A virus (A/Viet Nam/1203/2004(H5N1))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q6DQ18 (Residues: 339-512; Coverage: 31%)
Gene name: HA
Sequence domains: Haemagglutinin

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GAL, NGC
Carbohydrate polymer
1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P21
Unit cell:
a: 66.555Å b: 231.07Å c: 71.295Å
α: 90° β: 97.04° γ: 90°
R-values:
R R work R free
0.242 0.239 0.28
Expression system: Trichoplusia ni

Quick links

Citations

10 review citations

Molecular mechanisms and epidemiology of COVID-19 from an allergist's perspective.
Hosoki et al. (2020)
9 more

1 mention without citation

N-Glycolylneuraminic Acid as a Receptor for Influenza A Viruses.
Broszeit et al. (2019)