› 6e7k

X-ray diffraction
2.8Å resolution

Structure of the lipoprotein lipase GPIHBP1 complex that mediates plasma triglyceride hydrolysis

Released:
DOI: 10.2210/pdb6e7k/pdb
PDB entry 6e7k coloured by chain, front view.
PDB entry 6e7k coloured by chain, side view.
PDB entry 6e7k coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the lipoprotein lipase-GPIHBP1 complex that mediates plasma triglyceride hydrolysis.
Birrane G, Beigneux AP, Dwyer B, Strack-Logue B, Kristensen KK, Francone OL, Fong LG, Mertens HDT, Pan CQ, Ploug M, Young SG, Meiyappan M
Proc Natl Acad Sci U S A 116 1723-1732 (2019)
PMID: 30559189

Function and Biology Details

Reactions catalysed: 
Phosphatidylcholine + H(2)O = 2-acylglycerophosphocholine + a carboxylate
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139260 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (5 distinct):
Lipoprotein lipase Chains: A, B
Molecule details ›
Chains: A, B
Length: 448 amino acids
Theoretical weight: 50.38 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P06858 (Residues: 28-475; Coverage: 100%)
Gene names: LIPD, LPL
Sequence domains:
Glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 131 amino acids
Theoretical weight: 14.73 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8IV16 (Residues: 21-151; Coverage: 80%)
Gene names: GPIHBP1, HBP1
Sequence domains: u-PAR/Ly-6 domain

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
2 bound ligands:
Ligand CA 2 x CA
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P21212
Unit cell:
a: 101.948Å b: 153.206Å c: 95.783Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.195 0.235
Expression systems:
  • Homo sapiens
  • Spodoptera frugiperda

Quick links

Citations

11 review citations

Lipoprotein Lipase and Its Regulators: An Unfolding Story.
Wu et al. (2021)
10 more

14 mentions without citation

GPIHBP1 and Lipoprotein Lipase, Partners in Plasma Triglyceride Metabolism.
Young et al. (2019)
13 more