› 6e9e

Electron Microscopy
3.4Å resolution

EsCas13d-crRNA binary complex

Released:
DOI: 10.2210/pdb6e9e/pdb
PDB entry 6e9e coloured by chain, front view.
PDB entry 6e9e coloured by chain, side view.
PDB entry 6e9e coloured by chain, top view.
Source organisms:
Primary publication:
Structural Basis for the RNA-Guided Ribonuclease Activity of CRISPR-Cas13d.
Zhang C, Konermann S, Brideau NJ, Lotfy P, Wu X, Novick SJ, Strutzenberg T, Griffin PR, Hsu PD, Lyumkis D
Cell 175 212-223.e17 (2018)
PMID: 30241607
Related structures: EMD-9013

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109186 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
EsCas13d Chain: A
Molecule details ›
Chain: A
Length: 954 amino acids
Theoretical weight: 110.83 KDa
Source organism: [Eubacterium] siraeum DSM 15702
Expression system: Escherichia coli
UniProt:
  • Canonical: B0MS50 (Residues: 1-954; Coverage: 100%)
Gene name: EUBSIR_02687
crRNA (52-MER) Chain: B
Molecule details ›
Chain: B
Length: 51 nucleotides
Theoretical weight: 16.39 KDa
Source organism: bacterium
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-9013
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

41 review citations

Genome Editing with mRNA Encoding ZFN, TALEN, and Cas9.
Zhang et al. (2019)
40 more

6 mentions without citation

HEPN RNases - an emerging class of functionally distinct RNA processing and degradation enzymes.
Pillon et al. (2021)
5 more