› 6eaq

X-ray diffraction
2.22Å resolution

Glycosylated FCGR3B / CD16b in complex with afucosylated IgG1 Fc

Released:
DOI: 10.2210/pdb6eaq/pdb
PDB entry 6eaq coloured by chain, front view.
PDB entry 6eaq coloured by chain, side view.
PDB entry 6eaq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A single amino acid distorts the Fc γ receptor IIIb/CD16b structure upon binding immunoglobulin G1 and reduces affinity relative to CD16a.
Roberts JT, Barb AW
J Biol Chem 293 19899-19908 (2018)
PMID: 30361439

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130817 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (5 distinct):
Immunoglobulin gamma-1 heavy chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 220 amino acids
Theoretical weight: 24.81 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P0DOX5 (Residues: 227-446; Coverage: 49%)
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Low affinity immunoglobulin gamma Fc region receptor III-B Chain: C
Molecule details ›
Chain: C
Length: 175 amino acids
Theoretical weight: 20.05 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O75015 (Residues: 19-193; Coverage: 81%)
Gene names: CD16B, FCG3, FCGR3, FCGR3B, IGFR3
Sequence domains: Immunoglobulin domain
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN, GAL
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN, GAL
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2
Unit cell:
a: 123.629Å b: 49.388Å c: 139.415Å
α: 90° β: 103.04° γ: 90°
R-values:
R R work R free
0.215 0.213 0.244
Expression system: Homo sapiens

Quick links

Citations

4 review citations

Multiple Variables at the Leukocyte Cell Surface Impact Fc γ Receptor-Dependent Mechanisms.
Patel et al. (2019)
3 more

2 mentions without citation

A single amino acid distorts the Fc γ receptor IIIb/CD16b structure upon binding immunoglobulin G1 and reduces affinity relative to CD16a.
Roberts et al. (2018)
1 more