› 6ebd

X-ray diffraction
2.61Å resolution

OhrB (Organic Hydroperoxide Resistance protein) mutant (C60A) from Chromobacterium violaceum, interacting with dihydrolipoamide

Released:
DOI: 10.2210/pdb6ebd/pdb
PDB entry 6ebd coloured by chain, front view.
PDB entry 6ebd coloured by chain, side view.
PDB entry 6ebd coloured by chain, top view.
Source organism: Chromobacterium violaceum
Primary publication:
Substrate and product-assisted catalysis: molecular aspects behind structural switches along Organic Hydroperoxide Resistance Protein catalytic cycle
Domingos RM, Teixeira RD, Zeida A, Agudelo WA, Alegria TGP, da Silva Neto JF, Vieira PS, Murakami MT, Farah CS, Estrin DA, Netto LES
ACS Catalysis (2020)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103922 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Organic hydroperoxide resistance protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 161 amino acids
Theoretical weight: 16.82 KDa
Source organism: Chromobacterium violaceum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A202B6V5 (Residues: 1-141; Coverage: 100%)
Gene name: CBW21_15220
Sequence domains: OsmC-like protein

Ligands and Environments

2 bound ligands:
Ligand J3S 3 x J3S
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P61
Unit cell:
a: 87.675Å b: 87.675Å c: 179.609Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.194 0.232
Expression system: Escherichia coli BL21(DE3)

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