› 6ebq

X-ray diffraction
1.95Å resolution

Crystal Structure of the Flavoprotein NrdI from Aerococcus urinae in Oxidized Form

Released:
DOI: 10.2210/pdb6ebq/pdb
PDB entry 6ebq coloured by chain, front view.
PDB entry 6ebq coloured by chain, side view.
PDB entry 6ebq coloured by chain, top view.
Source organism: Aerococcus urinae
Primary publication:
Metal-free class Ie ribonucleotide reductase from pathogens initiates catalysis with a tyrosine-derived dihydroxyphenylalanine radical.
Blaesi EJ, Palowitch GM, Hu K, Kim AJ, Rose HR, Alapati R, Lougee MG, Kim HJ, Taguchi AT, Tan KO, Laremore TN, Griffin RG, Krebs C, Matthews ML, Silakov A, Bollinger JM, Allen BD, Boal AK
Proc Natl Acad Sci U S A 115 10022-10027 (2018)
PMID: 30224458

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123375 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein NrdI Chains: A, B
Molecule details ›
Chains: A, B
Length: 160 amino acids
Theoretical weight: 17.83 KDa
Source organism: Aerococcus urinae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F2I8Y0 (Residues: 2-142; Coverage: 99%)
Gene names: HMPREF9243_0732, nrdI
Sequence domains: NrdI Flavodoxin like
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand FMN 2 x FMN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 55.444Å b: 50.057Å c: 64.272Å
α: 90° β: 112.57° γ: 90°
R-values:
R R work R free
0.24 0.238 0.269
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

Ribonucleotide Reductases: Structure, Chemistry, and Metabolism Suggest New Therapeutic Targets.
Greene et al. (2020)
4 more

1 mention without citation

Metal-free class Ie ribonucleotide reductase from pathogens initiates catalysis with a tyrosine-derived dihydroxyphenylalanine radical.
Blaesi et al. (2018)