› 6edc

X-ray diffraction
2.71Å resolution

hcGAS-16bp dsDNA complex

Released:
DOI: 10.2210/pdb6edc/pdb
PDB entry 6edc coloured by chain, front view.
PDB entry 6edc coloured by chain, side view.
PDB entry 6edc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Human cGAS catalytic domain has an additional DNA-binding interface that enhances enzymatic activity and liquid-phase condensation.
Xie W, Lama L, Adura C, Tomita D, Glickman JF, Tuschl T, Patel DJ
Proc Natl Acad Sci U S A 116 11946-11955 (2019)
PMID: 31142647

Function and Biology Details

Reaction catalysed: 
(1a) ATP + GTP = pppG-P(2'-5')A + diphosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119839 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Cyclic GMP-AMP synthase Chain: A
Molecule details ›
Chain: A
Length: 366 amino acids
Theoretical weight: 42.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8N884 (Residues: 157-522; Coverage: 70%)
Gene names: C6orf150, CGAS, MB21D1
Sequence domains:
Structure domains:
DNA (5'-D(*AP*AP*AP*TP*TP*GP*CP*CP*GP*AP*AP*GP*AP*CP*GP*AP*A)-3') Chain: B
Molecule details ›
Chain: B
Length: 17 nucleotides
Theoretical weight: 5.25 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*TP*TP*CP*GP*TP*CP*TP*TP*CP*GP*GP*CP*AP*AP*T)-3') Chain: C
Molecule details ›
Chain: C
Length: 17 nucleotides
Theoretical weight: 5.16 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P6122
Unit cell:
a: 99.813Å b: 99.813Å c: 238.644Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.241 0.239 0.278
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

46 review citations

Molecular mechanisms and cellular functions of cGAS-STING signalling.
Hopfner et al. (2020)
45 more

1 mention without citation

Human cGAS catalytic domain has an additional DNA-binding interface that enhances enzymatic activity and liquid-phase condensation.
Xie et al. (2019)