› 6ee1

X-ray diffraction
2.36Å resolution

Crystal structure of Mycobacterium tuberculosis ICL2 in complex with acetyl-CoA

Released:
DOI: 10.2210/pdb6ee1/pdb
Model geometry
Fit model/data
PDB entry 6ee1 coloured by chain, front view.
PDB entry 6ee1 coloured by chain, side view.
PDB entry 6ee1 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis CDC1551
Primary publication:
Acetyl-CoA-mediated activation of Mycobacterium tuberculosis isocitrate lyase 2.
Bhusal RP, Jiao W, Kwai BXC, Reynisson J, Collins AJ, Sperry J, Bashiri G, Leung IKH
OpenAccess logo Nat Commun 10 4639 (2019)
PMID: 31604954

Function and Biology Details

Reaction catalysed: 
Isocitrate = succinate + glyoxylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186597 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isocitrate lyase 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 786 amino acids
Theoretical weight: 87.6 KDa
Source organism: Mycobacterium tuberculosis CDC1551
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8VJU4 (Residues: 1-766; Coverage: 100%)
Gene names: MT1966, aceA-2, icl2
Sequence domains: Isocitrate lyase family

Ligands and Environments

2 bound ligands:
Ligand ACO 4 x ACO
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P21
Unit cell:
a: 105.475Å b: 169.972Å c: 106.425Å
α: 90° β: 95.26° γ: 90°
R-values:
R R work R free
0.2 0.198 0.232
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Engineering the glyoxylate cycle for chemical bioproduction.
Yang et al. (2022)
7 other articles

1 mention without citation

The pathogenic mechanism of Mycobacterium tuberculosis: implication for new drug development.
Yan et al. (2022)