› 6eeb

X-ray diffraction
1.96Å resolution

Calmodulin in complex with malbrancheamide

Released:
DOI: 10.2210/pdb6eeb/pdb
PDB entry 6eeb coloured by chain, front view.
PDB entry 6eeb coloured by chain, side view.
PDB entry 6eeb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Perturbation of the interactions of calmodulin with GRK5 using a natural product chemical probe.
Beyett TS, Fraley AE, Labudde E, Patra D, Coleman RC, Eguchi A, Glukhova A, Chen Q, Williams RM, Koch WJ, Sherman DH, Tesmer JJG
Proc Natl Acad Sci U S A 116 15895-15900 (2019)
PMID: 31337679

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-143908 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calmodulin-1 Chain: A
Molecule details ›
Chain: A
Length: 152 amino acids
Theoretical weight: 17.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DP23 (Residues: 1-149; Coverage: 100%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair

Ligands and Environments

3 bound ligands:
Ligand J6P 1 x J6P
Ligand CA 4 x CA
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: C2221
Unit cell:
a: 49.113Å b: 56.88Å c: 116.781Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.214 0.263
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Impact of Aldosterone on the Failing Myocardium: Insights from Mitochondria and Adrenergic Receptors Signaling and Function.
Guitart-Mampel et al. (2021)
2 more

1 mention without citation

Perturbation of the interactions of calmodulin with GRK5 using a natural product chemical probe.
Beyett et al. (2019)