› 6eeq

X-ray diffraction
2.6Å resolution

Crystal structure of Rhodiola rosea 4-hydroxyphenylacetaldehyde synthase

Released:
DOI: 10.2210/pdb6eeq/pdb
PDB entry 6eeq coloured by chain, front view.
PDB entry 6eeq coloured by chain, side view.
PDB entry 6eeq coloured by chain, top view.
Source organism: Rhodiola rosea
Primary publication:
Structural basis for divergent and convergent evolution of catalytic machineries in plant aromatic amino acid decarboxylase proteins.
Torrens-Spence MP, Chiang YC, Smith T, Vicent MA, Wang Y, Weng JK
Proc Natl Acad Sci U S A 117 10806-10817 (2020)
PMID: 32371491

Function and Biology Details

Reaction catalysed: 
L-tyrosine + O(2) + H(2)O = (4-hydroxyphenyl)acetaldehyde + CO(2) + NH(3) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104258 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-hydroxyphenylacetaldehyde synthase Chain: A
Molecule details ›
Chain: A
Length: 490 amino acids
Theoretical weight: 54.66 KDa
Source organism: Rhodiola rosea
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A2I6B3P0 (Residues: 1-490; Coverage: 100%)
Gene names: 4HPAAS, HPAAS
Sequence domains: Pyridoxal-dependent decarboxylase conserved domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand LLP 1 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P43212
Unit cell:
a: 118.36Å b: 118.36Å c: 67.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.211 0.269
Expression system: Escherichia coli

Quick links

Citations

5 review citations

General and specialized tyrosine metabolism pathways in plants.
Xu et al. (2020)
4 more

1 mention without citation

Structural basis for divergent and convergent evolution of catalytic machineries in plant aromatic amino acid decarboxylase proteins.
Torrens-Spence et al. (2020)