› 6eew

X-ray diffraction
2.05Å resolution

Crystal structure of Catharanthus roseus tryptophan decarboxylase in complex with L-tryptophan

Released:
DOI: 10.2210/pdb6eew/pdb
PDB entry 6eew coloured by chain, front view.
PDB entry 6eew coloured by chain, side view.
PDB entry 6eew coloured by chain, top view.
Source organism: Catharanthus roseus
Primary publication:
Structural basis for divergent and convergent evolution of catalytic machineries in plant aromatic amino acid decarboxylase proteins.
Torrens-Spence MP, Chiang YC, Smith T, Vicent MA, Wang Y, Weng JK
Proc Natl Acad Sci U S A 117 10806-10817 (2020)
PMID: 32371491

Function and Biology Details

Reaction catalysed: 
5-hydroxy-L-tryptophan = 5-hydroxytryptamine + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148168 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aromatic-L-amino-acid decarboxylase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 500 amino acids
Theoretical weight: 56.51 KDa
Source organism: Catharanthus roseus
Expression system: Escherichia coli
UniProt:
  • Canonical: P17770 (Residues: 1-500; Coverage: 100%)
Gene name: TDC
Sequence domains: Pyridoxal-dependent decarboxylase conserved domain

Ligands and Environments

2 bound ligands:
Ligand TRP 4 x TRP
Ligand CA 4 x CA
1 modified residue:
Ligand LLP 4 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 107.612Å b: 69.607Å c: 133.472Å
α: 90° β: 93.778° γ: 90°
R-values:
R R work R free
0.221 0.22 0.256
Expression system: Escherichia coli

Quick links

Citations

5 review citations

General and specialized tyrosine metabolism pathways in plants.
Xu et al. (2020)
4 more

4 mentions without citation

Structural basis for divergent and convergent evolution of catalytic machineries in plant aromatic amino acid decarboxylase proteins.
Torrens-Spence et al. (2020)
3 more