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6en9

X-ray diffraction
1.5Å resolution

E. coli Hydrogenase-2 (hydrogen reduced form)

Released:
Model geometry
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Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-142265 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Hydrogenase-2 small chain Chains: S, T
Molecule details ›
Chains: S, T
Length: 298 amino acids
Theoretical weight: 32.37 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P69741 (Residues: 39-331; Coverage: 88%)
Gene names: JW2965, b2997, hybO, yghV
Sequence domains:
Structure domains:
Hydrogenase-2 large chain Chains: L, M
Molecule details ›
Chains: L, M
Length: 567 amino acids
Theoretical weight: 62.56 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACE0 (Residues: 1-567; Coverage: 100%)
Gene names: JW2962, b2994, hybC
Sequence domains: Nickel-dependent hydrogenase
Structure domains: Cytochrome-c3 Hydrogenase, chain B

Ligands and Environments

No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 99.602Å b: 100.54Å c: 168.587Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.153 0.152 0.175
Expression system: Escherichia coli