› 6ep2

X-ray diffraction
2.15Å resolution

Enterococcus faecalis FIC protein in complex with ADP and calcium ion.

Released:
DOI: 10.2210/pdb6ep2/pdb
PDB entry 6ep2 coloured by chain, front view.
PDB entry 6ep2 coloured by chain, side view.
PDB entry 6ep2 coloured by chain, top view.
Source organism: Enterococcus faecalis
Primary publication:
A Ca2+-regulated deAMPylation switch in human and bacterial FIC proteins.
Veyron S, Oliva G, Rolando M, Buchrieser C, Peyroche G, Cherfils J
Nat Commun 10 1142 (2019)
PMID: 30850593

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-220558 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fic family protein Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 207 amino acids
Theoretical weight: 24.64 KDa
Source organism: Enterococcus faecalis
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

2 bound ligands:
Ligand ADP 12 x ADP
Ligand CA 24 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P43212
Unit cell:
a: 125.311Å b: 125.311Å c: 362.879Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.187 0.218
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Inositol Requiring Enzyme (IRE), a multiplayer in sensing endoplasmic reticulum stress.
Zhou et al. (2021)
1 more

2 mentions without citation

A Ca2+-regulated deAMPylation switch in human and bacterial FIC proteins.
Veyron et al. (2019)
1 more