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› 6ess

X-ray diffraction
1.91Å resolution

Artificial imine reductase mutant S112A-N118P-K121A-S122M

Released:
DOI: 10.2210/pdb6ess/pdb
Model geometry
Fit model/data
PDB entry 6ess coloured by chain, front view.
PDB entry 6ess coloured by chain, side view.
PDB entry 6ess coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
Directed Evolution of an Artificial Imine Reductase.
Hestericová M, Heinisch T, Alonso-Cotchico L, Maréchal JD, Vidossich P, Ward TR
Angew Chem Int Ed Engl 57 1863-1868 (2018)
PMID: 29265726

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chain: A
Molecule details ›
Chain: A
Length: 159 amino acids
Theoretical weight: 16.52 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli
UniProt:
  • Canonical: P22629 (Residues: 38-183; Coverage: 92%)
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

2 bound ligands:
Ligand 4IR 1 x 4IR
Ligand IR 3 x IR
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X06DA
Spacegroup: I4122
Unit cell:
a: 58.079Å b: 58.079Å c: 185.125Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.278 0.276 0.327
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Artificial Metalloenzymes: Challenges and Opportunities.
Davis et al. (2019)
7 more

2 mentions without citation

Artificial Metalloenzymes Based on the Biotin-Streptavidin Technology: Enzymatic Cascades and Directed Evolution.
Liang et al. (2019)
1 more