› 6eyc

Electron Microscopy
3.8Å resolution

Re-refinement of the MCM2-7 double hexamer using ISOLDE

Released:
DOI: 10.2210/pdb6eyc/pdb
PDB entry 6eyc coloured by chain, front view.
PDB entry 6eyc coloured by chain, side view.
PDB entry 6eyc coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
ISOLDE: a physically realistic environment for model building into low-resolution electron-density maps.
Croll TI
OpenAccess logo Acta Crystallogr D Struct Biol 74 519-530 (2018)
PMID: 29872003
Related structures: EMD-6338

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150191 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
DNA replication licensing factor MCM2 Chain: 2
Molecule details ›
Chain: 2
Length: 868 amino acids
Theoretical weight: 98.91 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P29469 (Residues: 1-868; Coverage: 100%)
Gene names: MCM2, YBL023C, YBL0438
Sequence domains:
DNA replication licensing factor MCM3 Chain: 3
Molecule details ›
Chain: 3
Length: 971 amino acids
Theoretical weight: 107.65 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P24279 (Residues: 1-971; Coverage: 100%)
Gene names: MCM3, SYGP-ORF23, YEL032W
Sequence domains:
DNA replication licensing factor MCM4 Chain: 4
Minichromosome maintenance protein 5 Chain: 5
DNA replication licensing factor MCM6 Chain: 6
DNA replication licensing factor MCM7 Chain: 7

Ligands and Environments

2 bound ligands:
Ligand ZN 5 x ZN
Ligand ADP 6 x ADP
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.8Å
Relevant EMDB volumes: EMD-6338
Expression system: Saccharomyces cerevisiae S288C

Quick links

Citations

11 review citations

Better, Faster, Cheaper: Recent Advances in Cryo-Electron Microscopy.
Chua et al. (2022)
10 more

8 mentions without citation

Origins of DNA replication in eukaryotes.
Hu et al. (2023)
7 more