› 6f95

Electron Microscopy
3.44Å resolution

AlfA from B. subtilis plasmid pLS32 filament structure at 3.4 A

Released:
DOI: 10.2210/pdb6f95/pdb
PDB entry 6f95 coloured by chain, front view.
PDB entry 6f95 coloured by chain, side view.
PDB entry 6f95 coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Cryo-EM reconstruction of AlfA from Bacillus subtilis reveals the structure of a simplified actin-like filament at 3.4-Å resolution.
Szewczak-Harris A, Löwe J
Proc Natl Acad Sci U S A 115 3458-3463 (2018)
PMID: 29440489
Related structures: EMD-4196

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129419 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Actin-like protein N-terminal domain-containing protein Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 278 amino acids
Theoretical weight: 31.2 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: O52947 (Residues: 1-275; Coverage: 100%)
Sequence domains: Actin like proteins N terminal domain

Ligands and Environments

2 bound ligands:
Ligand MG 5 x MG
Ligand ADP 5 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.44Å
Relevant EMDB volumes: EMD-4196
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The Large pBS32/pLS32 Plasmid of Ancestral Bacillus subtilis.
Burton et al. (2020)
4 other articles

1 mention without citation

Cryo-EM reconstruction of AlfA from Bacillus subtilis reveals the structure of a simplified actin-like filament at 3.4-Å resolution.
Szewczak-Harris et al. (2018)