› 6fcq

X-ray diffraction
3.1Å resolution

The X-ray Structure of Lytic Transglycosylase Slt inactive mutant E503Q from Pseudomonas aeruginosa in complex with Bulgecin A

Released:
DOI: 10.2210/pdb6fcq/pdb
PDB entry 6fcq coloured by chain, front view.
PDB entry 6fcq coloured by chain, side view.
PDB entry 6fcq coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Exolytic and endolytic turnover of peptidoglycan by lytic transglycosylase Slt of Pseudomonas aeruginosa.
Lee M, Batuecas MT, Tomoshige S, Domínguez-Gil T, Mahasenan KV, Dik DA, Hesek D, Millán C, Usón I, Lastochkin E, Hermoso JA, Mobashery S
Proc Natl Acad Sci U S A 115 4393-4398 (2018)
PMID: 29632171

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191195 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable soluble lytic transglycosylase Chain: A
Molecule details ›
Chain: A
Length: 613 amino acids
Theoretical weight: 70.35 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HZI6 (Residues: 30-642; Coverage: 99%)
Gene name: PA3020
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand BUL 1 x BUL
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P63
Unit cell:
a: 165.715Å b: 165.715Å c: 55.884Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.181 0.217
Expression system: Escherichia coli

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Citations

2 review citations

Cell-Wall Recycling of the Gram-Negative Bacteria and the Nexus to Antibiotic Resistance.
Dik et al. (2018)
1 more

2 mentions without citation

Constructing and deconstructing the bacterial cell wall.
Fisher et al. (2020)
1 more