› 6fft

Hybrid

Neutron structure of human transthyretin (TTR) S52P mutant in complex with tafamidis at room temperature to 2A resolution (quasi-Laue)

Released:
DOI: 10.2210/pdb6fft/pdb
PDB entry 6fft coloured by chain, front view.
PDB entry 6fft coloured by chain, side view.
PDB entry 6fft coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A molecular mechanism for transthyretin amyloidogenesis.
Yee AW, Aldeghi M, Blakeley MP, Ostermann A, Mas PJ, Moulin M, de Sanctis D, Bowler MW, Mueller-Dieckmann C, Mitchell EP, Haertlein M, de Groot BL, Boeri Erba E, Forsyth VT
Nat Commun 10 925 (2019)
PMID: 30804345

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136168 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transthyretin Chains: A, B
Molecule details ›
Chains: A, B
Length: 130 amino acids
Theoretical weight: 14.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02766 (Residues: 21-147; Coverage: 100%)
Gene names: PALB, TTR
Sequence domains: HIUase/Transthyretin family

Ligands and Environments

1 bound ligand:
Ligand 3MI 2 x 3MI
No modified residues

Experiments and Validation Details

Experimental Method: X-ray diffraction, Neutron Diffraction
X-ray source: ILL BEAMLINE LADI III, ESRF BEAMLINE ID30B
Spacegroup: P21212
Unit cell:
a: 43.901Å b: 85.703Å c: 65.54Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.157 0.195
Expression system: Escherichia coli BL21(DE3)

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Citations

16 review citations

Higher-order structural characterisation of native proteins and complexes by top-down mass spectrometry.
Zhou et al. (2020)
15 more

1 mention without citation

A molecular mechanism for transthyretin amyloidogenesis.
Yee et al. (2019)