› 6fuw

Electron Microscopy
3.07Å resolution

Cryo-EM structure of the human CPSF160-WDR33-CPSF30 complex bound to the PAS AAUAAA motif at 3.1 Angstrom resolution

Released:
DOI: 10.2210/pdb6fuw/pdb
PDB entry 6fuw coloured by chain, front view.
PDB entry 6fuw coloured by chain, side view.
PDB entry 6fuw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of AAUAAA polyadenylation signal recognition by the human CPSF complex.
Clerici M, Faini M, Muckenfuss LM, Aebersold R, Jinek M
Nat Struct Mol Biol 25 135-138 (2018)
PMID: 29358758
Related structures: EMD-4225

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-131973 (preferred)
Entry contents:
3 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
Cleavage and polyadenylation specificity factor subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 1446 amino acids
Theoretical weight: 161.35 KDa
Source organism: Homo sapiens
Expression system: Spodoptera aff. frugiperda 2 RZ-2014
UniProt:
  • Canonical: Q10570 (Residues: 1-1443; Coverage: 100%)
Gene names: CPSF1, CPSF160
Sequence domains:
pre-mRNA 3' end processing protein WDR33 Chain: B
Molecule details ›
Chain: B
Length: 413 amino acids
Theoretical weight: 47.45 KDa
Source organism: Homo sapiens
Expression system: Spodoptera aff. frugiperda 2 RZ-2014
UniProt:
  • Canonical: Q9C0J8 (Residues: 1-410; Coverage: 31%)
Gene names: WDC146, WDR33
Sequence domains: WD domain, G-beta repeat
Cleavage and polyadenylation specificity factor subunit 4 Chain: C
Molecule details ›
Chain: C
Length: 178 amino acids
Theoretical weight: 20.42 KDa
Source organism: Homo sapiens
Expression system: Spodoptera aff. frugiperda 2 RZ-2014
UniProt:
  • Canonical: O95639 (Residues: 1-178; Coverage: 66%)
Gene names: CPSF30, CPSF4, NAR, NEB1
Sequence domains:
RNA (5'-R(P*AP*AP*UP*AP*AP*AP*GP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 10 nucleotides
Theoretical weight: 3.23 KDa

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.07Å
Relevant EMDB volumes: EMD-4225
Expression system: Spodoptera aff. frugiperda 2 RZ-2014

Quick links

Citations

16 review citations

Cryo-EM in drug discovery: achievements, limitations and prospects.
Renaud et al. (2018)
15 more

3 mentions without citation

Mechanistic insights into mRNA 3'-end processing.
Kumar et al. (2019)
2 more