› 6g2i

Electron Microscopy
5.9Å resolution

Filament of acetyl-CoA carboxylase and BRCT domains of BRCA1 (ACC-BRCT) at 5.9 A resolution

Released:
DOI: 10.2210/pdb6g2i/pdb
PDB entry 6g2i coloured by chain, front view.
PDB entry 6g2i coloured by chain, side view.
PDB entry 6g2i coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for regulation of human acetyl-CoA carboxylase.
Hunkeler M, Hagmann A, Stuttfeld E, Chami M, Guri Y, Stahlberg H, Maier T
Nature 558 470-474 (2018)
PMID: 29899443
Related structures: EMD-4344

Function and Biology Details

Reactions catalysed: 
ATP + acetyl-CoA + HCO(3)(-) = ADP + phosphate + malonyl-CoA
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153647 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Acetyl-CoA carboxylase 1 Chains: A, B, C, D, E, F, G, J, Q, R
Molecule details ›
Chains: A, B, C, D, E, F, G, J, Q, R
Length: 2346 amino acids
Theoretical weight: 265.95 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q13085 (Residues: 1-2346; Coverage: 100%)
Gene names: ACAC, ACACA, ACC1, ACCA
Sequence domains:
Breast cancer type 1 susceptibility protein Chains: H, K, M, O, S, U, W, Y
Molecule details ›
Chains: H, K, M, O, S, U, W, Y
Length: 240 amino acids
Theoretical weight: 27.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P38398 (Residues: 1646-1859; Coverage: 12%)
Gene names: BRCA1, RNF53
Sequence domains: BRCA1 C Terminus (BRCT) domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SEP 10 x SEP

Experiments and Validation Details

Entry percentile scores
Resolution: 5.9Å
Relevant EMDB volumes: EMD-4344
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

28 review citations

Lipid metabolism and cancer.
Bian et al. (2021)
27 more

3 mentions without citation

Therapeutic Targeting the Allosteric Cysteinome of RAS and Kinase Families.
Li et al. (2022)
2 more