› 6gk4

X-ray diffraction
2.91Å resolution

Human NBD1 of CFTR in complex with nanobodies D12 and T8

Released:
DOI: 10.2210/pdb6gk4/pdb
Model geometry
Fit model/data
PDB entry 6gk4 coloured by chain, front view.
PDB entry 6gk4 coloured by chain, side view.
PDB entry 6gk4 coloured by chain, top view.
Source organisms:
Primary publication:
Domain-interface dynamics of CFTR revealed by stabilizing nanobodies.
Sigoillot M, Overtus M, Grodecka M, Scholl D, Garcia-Pino A, Laeremans T, He L, Pardon E, Hildebrandt E, Urbatsch I, Steyaert J, Riordan JR, Govaerts C
OpenAccess logo Nat Commun 10 2636 (2019)
PMID: 31201318

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + closed Cl(-) channel = ADP + phosphate + open Cl(-) channel
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210547 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cystic fibrosis transmembrane conductance regulator Chains: A, D
Molecule details ›
Chains: A, D
Length: 229 amino acids
Theoretical weight: 25.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P13569 (Residues: 387-646; Coverage: 15%)
Gene names: ABCC7, CFTR
Sequence domains: ABC transporter
Nanobody D12 Chains: B, E
Molecule details ›
Chains: B, E
Length: 149 amino acids
Theoretical weight: 16.27 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Nanobody T8 Chains: C, F

Ligands and Environments

3 bound ligands:
Ligand GOL 1 x GOL
Ligand MG 2 x MG
Ligand ATP 2 x ATP
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 80.94Å b: 55.19Å c: 114.99Å
α: 90° β: 103.96° γ: 90°
R-values:
R R work R free
0.227 0.246 0.295
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Accelerating GPCR Drug Discovery With Conformation-Stabilizing VHHs.
Laeremans et al. (2022)
2 more

2 mentions without citation

Recent Strategic Advances in CFTR Drug Discovery: An Overview.
Rusnati et al. (2020)
1 more