› 6goe

X-ray diffraction
1.6Å resolution

KRAS full length G12V GPPNHP

Released:
DOI: 10.2210/pdb6goe/pdb
Model geometry
Fit model/data
PDB entry 6goe coloured by chain, front view.
PDB entry 6goe coloured by chain, side view.
PDB entry 6goe coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure-based development of new RAS-effector inhibitors from a combination of active and inactive RAS-binding compounds.
Cruz-Migoni A, Canning P, Quevedo CE, Bataille CJR, Bery N, Miller A, Russell AJ, Phillips SEV, Carr SB, Rabbitts TH
OpenAccess logo Proc Natl Acad Sci U S A 116 2545-2550 (2019)
PMID: 30683716

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133991 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase KRas, N-terminally processed Chain: A
Molecule details ›
Chain: A
Length: 172 amino acids
Theoretical weight: 19.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01116 (Residues: 2-169; Coverage: 89%)
  • Best match: P01116-2 (Residues: 2-171)
Gene names: KRAS, KRAS2, RASK2
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand GNP 1 x GNP
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: R3
Unit cell:
a: 79.88Å b: 79.88Å c: 78.79Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.182 0.22
Expression system: Escherichia coli

Quick links

Citations

20 review citations

RAS-targeted therapies: is the undruggable drugged?
Moore et al. (2020)
19 more

3 mentions without citation

Structure-based inhibitor design of mutant RAS proteins-a paradigm shift.
Nyíri et al. (2020)
2 more