› 6gx9

X-ray diffraction
2.7Å resolution

Crystal structure of the TNPO3 - CPSF6 RSLD complex

Released:
DOI: 10.2210/pdb6gx9/pdb
PDB entry 6gx9 coloured by chain, front view.
PDB entry 6gx9 coloured by chain, side view.
PDB entry 6gx9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Differential role for phosphorylation in alternative polyadenylation function versus nuclear import of SR-like protein CPSF6.
Jang S, Cook NJ, Pye VE, Bedwell GJ, Dudek AM, Singh PK, Cherepanov P, Engelman AN
Nucleic Acids Res 47 4663-4683 (2019)
PMID: 30916345

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172534 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transportin-3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 923 amino acids
Theoretical weight: 104.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y5L0 (Residues: 1-923; Coverage: 100%)
Gene names: IPO12, TNPO3
Sequence domains: Exportin 1-like protein
Cleavage and polyadenylation specificity factor subunit 6 Chains: C, D
Molecule details ›
Chains: C, D
Length: 70 amino acids
Theoretical weight: 9.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16630 (Residues: 481-550; Coverage: 13%)
Gene names: CFIM68, CPSF6

Ligands and Environments

3 bound ligands:
Ligand MG 4 x MG
Ligand BCN 2 x BCN
Ligand BEN 2 x BEN
1 modified residue:
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P6322
Unit cell:
a: 200.335Å b: 200.335Å c: 234.807Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.215 0.214 0.248
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Karyopherin-mediated nucleocytoplasmic transport.
Wing et al. (2022)
10 more

1 mention without citation

Differential role for phosphorylation in alternative polyadenylation function versus nuclear import of SR-like protein CPSF6.
Jang et al. (2019)