› 6gy2

X-ray diffraction
3.11Å resolution

Crystal structure of human Plk1-PBD in complex with WSSSLATPPTLSSpTVLI phosphopeptide from BRCA2

Released:
DOI: 10.2210/pdb6gy2/pdb
PDB entry 6gy2 coloured by chain, front view.
PDB entry 6gy2 coloured by chain, side view.
PDB entry 6gy2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Proper chromosome alignment depends on BRCA2 phosphorylation by PLK1.
Ehlén Å, Martin C, Miron S, Julien M, Theillet FX, Ropars V, Sessa G, Beaurepere R, Boucherit V, Duchambon P, El Marjou A, Zinn-Justin S, Carreira A
Nat Commun 11 1819 (2020)
PMID: 32286328

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156370 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein kinase PLK1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 245 amino acids
Theoretical weight: 28.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P53350 (Residues: 365-603; Coverage: 40%)
Gene names: PLK, PLK1
Sequence domains: POLO box duplicated region
Breast cancer type 2 susceptibility protein Chains: C, D
Molecule details ›
Chains: C, D
Length: 17 amino acids
Theoretical weight: 1.84 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P51587 (Residues: 194-210; Coverage: 1%)
Gene names: BRCA2, FACD, FANCD1

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
1 modified residue:
Ligand TPO 2 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P1
Unit cell:
a: 50Å b: 56.04Å c: 61.03Å
α: 80.79° β: 79.23° γ: 65.05°
R-values:
R R work R free
0.189 0.187 0.215
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

10 review citations

Shaping the BRCAness mutational landscape by alternative double-strand break repair, replication stress and mitotic aberrancies.
Stok et al. (2021)
9 more