› 6gyu

Electron Microscopy
3Å resolution

Cryo-EM structure of the CBF3-msk complex of the budding yeast kinetochore

Released:
DOI: 10.2210/pdb6gyu/pdb
PDB entry 6gyu coloured by chain, front view.
PDB entry 6gyu coloured by chain, side view.
PDB entry 6gyu coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Architecture of the CBF3-centromere complex of the budding yeast kinetochore.
Yan K, Zhang Z, Yang J, McLaughlin SH, Barford D
Nat Struct Mol Biol 25 1103-1110 (2018)
PMID: 30478265
Related structures: EMD-0097

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-152337 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Centromere DNA-binding protein complex CBF3 subunit B Chain: B
Molecule details ›
Chain: B
Length: 608 amino acids
Theoretical weight: 71.44 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P40969 (Residues: 1-608; Coverage: 100%)
Gene names: CBF3, CBF3B, CEP3, CSL1, YM8520.17C, YMR168C
Sequence domains:
Centromere DNA-binding protein complex CBF3 subunit C Chain: A
Molecule details ›
Chain: A
Length: 478 amino acids
Theoretical weight: 56.42 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P35203 (Residues: 1-478; Coverage: 100%)
Gene names: CBF3C, CTF13, YM6543.01, YM9582.19, YMR094W
Centromere DNA-binding protein complex CBF3 subunit B Chain: C
Molecule details ›
Chain: C
Length: 564 amino acids
Theoretical weight: 66.4 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P40969 (Residues: 49-608; Coverage: 92%)
Gene names: CBF3, CBF3B, CEP3, CSL1, YM8520.17C, YMR168C
Sequence domains: Centromere DNA-binding protein complex CBF3 subunit B
Suppressor of kinetochore protein 1 Chain: D
Molecule details ›
Chain: D
Length: 194 amino acids
Theoretical weight: 22.36 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P52286 (Residues: 1-194; Coverage: 100%)
Gene names: CBF3D, D9798.14, SKP1, YDR328C
Sequence domains:
Centromere DNA-binding protein complex CBF3 subunit A Chain: E
Molecule details ›
Chain: E
Length: 956 amino acids
Theoretical weight: 112.07 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P32504 (Residues: 1-956; Coverage: 100%)
Gene names: CBF2, CBF3A, CEP2, CTF14, NDC10, YGR140W
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-0097
Expression system: unidentified baculovirus

Quick links

Citations

4 review citations

Seeing is believing: our evolving view of kinetochore structure, composition, and assembly.
Hamilton et al. (2019)
3 more

1 mention without citation

Architecture of the CBF3-centromere complex of the budding yeast kinetochore.
Yan et al. (2018)