› 6h1m

Neutron Diffraction
2.15Å resolution

Neutron structure of Lactobacillus brevis alcohol dehydrogenase

Released:
DOI: 10.2210/pdb6h1m/pdb
PDB entry 6h1m coloured by chain, front view.
PDB entry 6h1m coloured by chain, side view.
PDB entry 6h1m coloured by chain, top view.
Source organism: Levilactobacillus brevis
Primary publication:
Neutron and X-ray crystal structures of Lactobacillus brevis alcohol dehydrogenase reveal new insights into hydrogen-bonding pathways.
Hermann J, Nowotny P, Schrader TE, Biggel P, Hekmat D, Weuster-Botz D
Acta Crystallogr F Struct Biol Commun 74 754-764 (2018)
PMID: 30511668

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183030 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
R-specific alcohol dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 251 amino acids
Theoretical weight: 26.66 KDa
Source organism: Levilactobacillus brevis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q84EX5 (Residues: 2-252; Coverage: 100%)
Gene name: radh
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand MN 1 x MN
No modified residues

Experiments and Validation Details

Spacegroup: I222
Unit cell:
a: 56.508Å b: 84.572Å c: 115.419Å α: 90° β: 90° γ: 90°
R-values:
R R work R free 0.221 0.218 0.249
Expression system: Escherichia coli BL21(DE3)

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Citations

2 mentions without citation

Neutron and X-ray crystal structures of Lactobacillus brevis alcohol dehydrogenase reveal new insights into hydrogen-bonding pathways.
Hermann et al. (2018)
1 more