› 6h25

Electron Microscopy
3.8Å resolution

Human nuclear RNA exosome EXO-10-MPP6 complex

Released:
DOI: 10.2210/pdb6h25/pdb
PDB entry 6h25 coloured by chain, front view.
PDB entry 6h25 coloured by chain, side view.
PDB entry 6h25 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Distinct and evolutionary conserved structural features of the human nuclear exosome complex.
Gerlach P, Schuller JM, Bonneau F, Basquin J, Reichelt P, Falk S, Conti E
Elife 7 (2018)
PMID: 30047866
Related structures: EMD-0128

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170318 (preferred)
Entry contents:
11 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (12 distinct):
Exosome complex component RRP45 Chain: A
Molecule details ›
Chain: A
Length: 443 amino acids
Theoretical weight: 49.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q06265 (Residues: 1-439; Coverage: 100%)
Gene names: EXOSC9, PMSCL1
Sequence domains:
Structure domains: GHMP Kinase, N-terminal domain
Exosome complex component RRP41 Chain: B
Molecule details ›
Chain: B
Length: 249 amino acids
Theoretical weight: 26.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NPD3 (Residues: 1-245; Coverage: 100%)
Gene names: EXOSC4, RRP41, SKI6
Sequence domains:
Structure domains: GHMP Kinase, N-terminal domain
Exosome complex component RRP43 Chain: C
Molecule details ›
Chain: C
Length: 278 amino acids
Theoretical weight: 30.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96B26 (Residues: 1-276; Coverage: 100%)
Gene names: EXOSC8, OIP2, RRP43
Sequence domains:
Structure domains: GHMP Kinase, N-terminal domain
Exosome complex component RRP46 Chain: D
Molecule details ›
Chain: D
Length: 237 amino acids
Theoretical weight: 25.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NQT4 (Residues: 1-235; Coverage: 100%)
Gene names: CML28, EXOSC5, RRP46
Sequence domains:
Structure domains: GHMP Kinase, N-terminal domain
Exosome complex component RRP42 Chain: E
Molecule details ›
Chain: E
Length: 295 amino acids
Theoretical weight: 32.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15024 (Residues: 1-291; Coverage: 100%)
Gene names: EXOSC7, KIAA0116, RRP42
Sequence domains:
Structure domains: GHMP Kinase, N-terminal domain
Exosome complex component MTR3 Chain: F
Molecule details ›
Chain: F
Length: 276 amino acids
Theoretical weight: 28.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5RKV6 (Residues: 1-272; Coverage: 100%)
Gene names: EXOSC6, MTR3
Sequence domains: 3' exoribonuclease family, domain 1
Exosome complex component RRP40 Chain: G
Molecule details ›
Chain: G
Length: 293 amino acids
Theoretical weight: 30.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NQT5 (Residues: 1-275; Coverage: 100%)
Gene names: CGI-102, EXOSC3, RRP40
Sequence domains:
Exosome complex component RRP4 Chain: H
Molecule details ›
Chain: H
Length: 297 amino acids
Theoretical weight: 33.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13868 (Residues: 1-293; Coverage: 100%)
Gene names: EXOSC2, RRP4
Sequence domains:
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Exosome complex component CSL4 Chain: I
Molecule details ›
Chain: I
Length: 199 amino acids
Theoretical weight: 21.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y3B2 (Residues: 1-195; Coverage: 100%)
Gene names: CGI-108, CSL4, EXOSC1
Sequence domains:
Exosome complex exonuclease RRP44 Chain: J
Molecule details ›
Chain: J
Length: 962 amino acids
Theoretical weight: 109.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y2L1 (Residues: 1-958; Coverage: 100%)
Gene names: DIS3, KIAA1008, RRP44
Sequence domains:
M-phase phosphoprotein 6 Chain: K
Molecule details ›
Chain: K
Length: 166 amino acids
Theoretical weight: 19.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99547 (Residues: 1-160; Coverage: 100%)
Gene names: MPHOSPH6, MPP6
Sequence domains: M-phase phosphoprotein 6
U44 ssRNA Chain: R
Molecule details ›
Chain: R
Length: 44 nucleotides
Theoretical weight: 13.43 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-0128
Expression system: Escherichia coli

Quick links

Citations

5 review citations

RNA helicases are hubs that orchestrate exosome-dependent 3'-5' decay.
Weick et al. (2021)
4 more

5 mentions without citation

Distinct and evolutionary conserved structural features of the human nuclear exosome complex.
Gerlach et al. (2018)
4 more