6h3c

Electron Microscopy
3.9Å resolution

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-152740 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
BRISC complex subunit Abraxas 2 Chains: A, F
Molecule details ›
Chains: A, F
Length: 434 amino acids
Theoretical weight: 49.26 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q15018 (Residues: 1-415; Coverage: 100%)
Gene names: ABRAXAS2, ABRO1, FAM175B, KIAA0157
Sequence domains: BRCA1-A complex subunit Abraxas 1 MPN domain
Lys-63-specific deubiquitinase BRCC36 Chains: B, G
Molecule details ›
Chains: B, G
Length: 335 amino acids
Theoretical weight: 38.42 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P46736 (Residues: 1-316; Coverage: 100%)
Gene names: BRCC3, BRCC36, C6.1A, CXorf53
Sequence domains:
BRISC and BRCA1-A complex member 2 Chains: C, H
Molecule details ›
Chains: C, H
Length: 402 amino acids
Theoretical weight: 45.89 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9NXR7 (Residues: 1-383; Coverage: 100%)
Gene names: BABAM2, BRCC45, BRE
Sequence domains: Brain and reproductive organ-expressed protein (BRE)
BRISC and BRCA1-A complex member 1 Chains: D, I
Molecule details ›
Chains: D, I
Length: 352 amino acids
Theoretical weight: 39.26 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9NWV8 (Residues: 1-329; Coverage: 100%)
Gene names: BABAM1, C19orf62, HSPC142, MERIT40, NBA1
Serine hydroxymethyltransferase, mitochondrial Chains: E, J
Molecule details ›
Chains: E, J
Length: 507 amino acids
Theoretical weight: 56.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P34897 (Residues: 17-504; Coverage: 97%)
Gene name: SHMT2
Sequence domains: Serine hydroxymethyltransferase

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-0132
Expression systems:
  • Trichoplusia ni
  • Escherichia coli BL21(DE3)