› 6h45

X-ray diffraction
2.4Å resolution

crystal structure of the human TGT catalytic subunit QTRT1 in complex with queuine

Released:
DOI: 10.2210/pdb6h45/pdb
PDB entry 6h45 coloured by chain, front view.
PDB entry 6h45 coloured by chain, side view.
PDB entry 6h45 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal Structure of the Human tRNA Guanine Transglycosylase Catalytic Subunit QTRT1.
Johannsson S, Neumann P, Ficner R
Biomolecules 8 (2018)
PMID: 30149595

Function and Biology Details

Reaction catalysed: 
Guanine(34) in tRNA + queuine = queuine(34) in tRNA + guanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189797 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Queuine tRNA-ribosyltransferase catalytic subunit 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 392 amino acids
Theoretical weight: 43.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BXR0 (Residues: 12-403; Coverage: 97%)
Gene names: QTRT1, TGT, TGUT
Sequence domains: Queuine tRNA-ribosyltransferase

Ligands and Environments

6 bound ligands:
Ligand QEI 2 x QEI
Ligand ZN 2 x ZN
Ligand BR 4 x BR
Ligand CL 5 x CL
Ligand K 2 x K
Ligand GLU 1 x GLU
1 modified residue:
Ligand OCS 2 x OCS

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: C2
Unit cell:
a: 127.5Å b: 158.86Å c: 47.62Å
α: 90° β: 108.19° γ: 90°
R-values:
R R work R free
0.203 0.201 0.24
Expression system: Escherichia coli

Quick links

Citations

7 citation in other articles

Queuine Is a Nutritional Regulator of Entamoeba histolytica Response to Oxidative Stress and a Virulence Attenuator.
Nagaraja et al. (2021)
6 more

4 mentions without citation

Crystal Structure of the Human tRNA Guanine Transglycosylase Catalytic Subunit QTRT1.
Johannsson et al. (2018)
3 more