› 6h5q

Electron Microscopy
3.3Å resolution

Cryo-EM structure of in vitro assembled Measles virus N into nucleocapsid-like particles (NCLPs) bound to polyA RNA hexamers.

Released:
DOI: 10.2210/pdb6h5q/pdb
PDB entry 6h5q coloured by chain, front view.
PDB entry 6h5q coloured by chain, side view.
PDB entry 6h5q coloured by chain, top view.
Source organisms:
Primary publication:
Assembly and cryo-EM structures of RNA-specific measles virus nucleocapsids provide mechanistic insight into paramyxoviral replication.
Desfosses A, Milles S, Jensen MR, Guseva S, Colletier JP, Maurin D, Schoehn G, Gutsche I, Ruigrok RWH, Blackledge M
Proc Natl Acad Sci U S A 116 4256-4264 (2019)
PMID: 30787192
Related structures: EMD-0141

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
hetero 30-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110755 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Nucleocapsid Chain: B
Molecule details ›
Chain: B
Length: 415 amino acids
Theoretical weight: 46.43 KDa
Source organism: Measles morbillivirus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B8PZP0 (Residues: 1-405; Coverage: 77%)
Gene name: N
Sequence domains: Paramyxovirus nucleocapsid protein
RNA (5'-R(*AP*AP*AP*AP*AP*A)-3') Chain: R
Molecule details ›
Chain: R
Length: 6 nucleotides
Theoretical weight: 1.93 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-0141
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

7 review citations

Templated folding of intrinsically disordered proteins.
Toto et al. (2020)
6 more

4 mentions without citation

The Nucleocapsid of Paramyxoviruses: Structure and Function of an Encapsidated Template.
Bloyet LM. (2021)
3 more