› 6hbu

Electron Microscopy
3.09Å resolution

Cryo-EM structure of the ABCG2 E211Q mutant bound to ATP and Magnesium

Released:
DOI: 10.2210/pdb6hbu/pdb
PDB entry 6hbu coloured by chain, front view.
PDB entry 6hbu coloured by chain, side view.
PDB entry 6hbu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cryo-EM structures of a human ABCG2 mutant trapped in ATP-bound and substrate-bound states.
Manolaridis I, Jackson SM, Taylor NMI, Kowal J, Stahlberg H, Locher KP
Nature 563 426-430 (2018)
PMID: 30405239
Related structures: EMD-0190

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + xenobiotic(Side 1) = ADP + phosphate + xenobiotic(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194005 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Broad substrate specificity ATP-binding cassette transporter ABCG2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 655 amino acids
Theoretical weight: 72.38 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9UNQ0 (Residues: 1-655; Coverage: 100%)
Gene names: ABCG2, ABCP, BCRP, BCRP1, MXR
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ATP 2 x ATP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.09Å
Relevant EMDB volumes: EMD-0190
Expression system: Homo sapiens

Quick links

Citations

16 review citations

Structures of ABC transporters: handle with care.
Lewinson et al. (2020)
15 more

32 mentions without citation

Structural and functional diversity calls for a new classification of ABC transporters.
Thomas et al. (2020)
31 more