› 6hl6

X-ray diffraction
1.97Å resolution

Factor Inhibiting HIF (FIH) in complex with zinc, NOG and iASPP(670-693)

Released:
DOI: 10.2210/pdb6hl6/pdb
PDB entry 6hl6 coloured by chain, front view.
PDB entry 6hl6 coloured by chain, side view.
PDB entry 6hl6 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Leissing TM, Chowdhury R, Clifton IJ, Lu X, Schofield CJ

Function and Biology Details

Reactions catalysed: 
Hypoxia-inducible factor-L-asparagine + 2-oxoglutarate + O(2) = hypoxia-inducible factor-(3S)-3-hydroxy-L-asparagine + succinate + CO(2)
[Ankyrin-repeat domain protein]-L-asparagine + 2-oxoglutarate + O(2) = [ankyrin-repeat domain protein]-(3S)-3-hydroxy-L-asparagine + succinate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186714 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Hypoxia-inducible factor 1-alpha inhibitor Chain: A
Molecule details ›
Chain: A
Length: 350 amino acids
Theoretical weight: 40.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NWT6 (Residues: 1-349; Coverage: 100%)
Gene names: FIH1, HIF1AN
Sequence domains: Cupin-like domain
RelA-associated inhibitor Chain: S
Molecule details ›
Chain: S
Length: 24 amino acids
Theoretical weight: 2.42 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q8WUF5 (Residues: 670-693; Coverage: 3%)
Gene names: IASPP, NKIP1, PPP1R13BL, PPP1R13L, RAI

Ligands and Environments

4 bound ligands:
Ligand GOL 5 x GOL
Ligand SO4 3 x SO4
Ligand ZN 1 x ZN
Ligand OGA 1 x OGA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P41212
Unit cell:
a: 86.084Å b: 86.084Å c: 148.752Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.171 0.206
Expression systems:
  • Escherichia coli
  • Not provided

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