› 6hla

X-ray diffraction
1.9Å resolution

wild-type NuoEF from Aquifex aeolicus - reduced form bound to NADH

Released:
DOI: 10.2210/pdb6hla/pdb
Model geometry
Fit model/data
PDB entry 6hla coloured by chain, front view.
PDB entry 6hla coloured by chain, side view.
PDB entry 6hla coloured by chain, top view.
Source organism: Aquifex aeolicus VF5
Primary publication:
A mechanism to prevent production of reactive oxygen species by Escherichia coli respiratory complex I.
Schulte M, Frick K, Gnandt E, Jurkovic S, Burschel S, Labatzke R, Aierstock K, Fiegen D, Wohlwend D, Gerhardt S, Einsle O, Friedrich T
OpenAccess logo Nat Commun 10 2551 (2019)
PMID: 31186428

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130389 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NADH-quinone oxidoreductase subunit E Chains: A, C
Molecule details ›
Chains: A, C
Length: 160 amino acids
Theoretical weight: 18.57 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli
UniProt:
  • Canonical: O66842 (Residues: 1-160; Coverage: 100%)
Gene names: aq_574, nuoE
Sequence domains: Thioredoxin-like [2Fe-2S] ferredoxin
NADH-quinone oxidoreductase subunit F Chains: B, D
Molecule details ›
Chains: B, D
Length: 434 amino acids
Theoretical weight: 48.52 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli
UniProt:
  • Canonical: O66841 (Residues: 1-426; Coverage: 100%)
Gene names: aq_573, nuoF
Sequence domains:

Ligands and Environments

6 bound ligands:
Ligand FES 2 x FES
Ligand SF4 2 x SF4
Ligand FMN 2 x FMN
Ligand NAD 2 x NAD
Ligand NA 2 x NA
Ligand MPO 2 x MPO
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 63.642Å b: 116.431Å c: 189.452Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.215 0.24
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Mitochondrial iron-sulfur clusters: Structure, function, and an emerging role in vascular biology.
Read et al. (2021)
4 more

1 mention without citation

Structure of Escherichia coli respiratory complex I reconstituted into lipid nanodiscs reveals an uncoupled conformation.
Kolata et al. (2021)