› 6hn4

Electron Microscopy
4.2Å resolution

Leucine-zippered human insulin receptor ectodomain with single bound insulin - "lower" membrane-proximal part

Released:
DOI: 10.2210/pdb6hn4/pdb
PDB entry 6hn4 coloured by chain, front view.
PDB entry 6hn4 coloured by chain, side view.
PDB entry 6hn4 coloured by chain, top view.
Source organisms:
Primary publication:
The signalling conformation of the insulin receptor ectodomain.
Weis F, Menting JG, Margetts MB, Chan SJ, Xu Y, Tennagels N, Wohlfart P, Langer T, Müller CW, Dreyer MK, Lawrence MC
Nat Commun 9 4420 (2018)
PMID: 30356040
Related structures: EMD-0246

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136504 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
General control transcription factor GCN4; Insulin receptor subunit alpha Chains: E, F
Molecule details ›
Chains: E, F
Length: 930 amino acids
Theoretical weight: 106.73 KDa
Source organisms: Expression system: Cricetulus griseus
UniProt:
  • Canonical: P06213 (Residues: 28-955; Coverage: 66%)
  • Canonical: P03069 (Residues: 249-281; Coverage: 12%)
Gene names: AAS101, AAS3, ARG9, GCN4, INSR, YEL009C
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand NAG 6 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.2Å
Relevant EMDB volumes: EMD-0246
Expression system: Cricetulus griseus

Quick links

Citations

15 review citations

Receptor tyrosine kinase activation: From the ligand perspective.
Trenker et al. (2020)
14 more

10 mentions without citation

Insulin and epidermal growth factor receptor family members share parallel activation mechanisms.
Ferguson et al. (2020)
9 more